Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/19

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(Autocreate 2014/05/19 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (18:03, 19 May 2014) (view source)
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10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.  
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<br><br>
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Golden gate ligations for other receivers with 10:1 insert to bb ratio
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Reaction'''
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| align="center" style="background:#f0f0f0;"|'''Rec Bb'''
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| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
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| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
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| align="center" style="background:#f0f0f0;"|'''BsmBI'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
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| align="center" style="background:#f0f0f0;"|'''H2O'''
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|-
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| Bb ctrl||0.74||0||0||0.5||1.25||2||5.51
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|-
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| Rhl Rec||0.74||2.78||1.68||0.5||1.25||2||1.05
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|-
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| Esa Rec||0.74||4.35||2.7||0.5||1.25||2||-1.54
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|}
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mm/dd/yyyy

10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.

Golden gate ligations for other receivers with 10:1 insert to bb ratio

Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl0.74000.51.2525.51
Rhl Rec0.742.781.680.51.2521.05
Esa Rec0.744.352.70.51.252-1.54


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