Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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(mm/dd/yyyy)
Current revision (13:25, 15 May 2014) (view source)
(mm/dd/yyyy)
 
Line 27: Line 27:
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
 +
|-
 +
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
|-
|-
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
Line 33: Line 35:
|-
|-
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
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|-
 
-
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
 
|}
|}
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mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec56.995277.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl7.4000.51.2528.85
Lux Rec7.42.942.250.51.2523.66
Rhl Rec7.42.781.680.51.2524.39
Esa Rec7.44.352.70.51.2521.8


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