Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15: Difference between revisions

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|-
|-
| Receiver int vec||56.995||27||7.407407407
| Receiver int vec||56.995||27||7.407407407
|}
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Reaction'''
| align="center" style="background:#f0f0f0;"|'''Rec bb'''
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
| align="center" style="background:#f0f0f0;"|'''T4 ligase'''
| align="center" style="background:#f0f0f0;"|'''T4 ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
|-
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||1.54
|-
| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||2.27
|-
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||-0.32
|-
| Bb control||7.4||0||0||0.5||1.25||2||6.73
|}
|}
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Revision as of 10:03, 15 May 2014

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mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec 56.995 27 7.407407407


Reaction Rec bb pLux-GFP pLac-LuxR BsmBI T4 ligase T4 ligase buffer H2O
Lux Rec 7.4 2.94 2.25 0.5 1.25 2 1.54
Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 2.27
Esa Rec 7.4 4.35 2.7 0.5 1.25 2 -0.32
Bb control 7.4 0 0 0.5 1.25 2 6.73


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