Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/12

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(Autocreate 2014/05/12 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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Ran PCR products from 5/6/14 on a gel. Only 6, RhlI worked. <br><br>
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Re-ran other PCRs, annealing temp 40, 4min ext
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''F Primer'''
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| align="center" style="background:#f0f0f0;"|'''R Primer'''
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| align="center" style="background:#f0f0f0;"|'''Annealing T'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
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|-
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| 1||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
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|-
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| 2||LuxI sender const 8/31/13 179ng||P063||P064||23||654
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|-
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| 3||K084007 3/10/13 4-2-1||P069||P070||32||645
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|-
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| 4||K084007 3/10/13 4-2-2||P069||P070||32||645
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|-
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| 5||RBS-LasI 3/8/13 31ng||P069||P070||32||645
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|}
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<br>
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Ran a second one of the backbone with annealing temp 50, ext time 4 min
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Label'''
 +
| align="center" style="background:#f0f0f0;"|'''Template'''
 +
| align="center" style="background:#f0f0f0;"|'''F Primer'''
 +
| align="center" style="background:#f0f0f0;"|'''R Primer'''
 +
| align="center" style="background:#f0f0f0;"|'''Annealing T'''
 +
| align="center" style="background:#f0f0f0;"|'''Expected length'''
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|-
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| mCh sender||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
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|}
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<br><br>
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Ran on gel, only 5, LasI worked.
 +
<br><br>
 +
Ran a third, annealing temp 30
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Label'''
 +
| align="center" style="background:#f0f0f0;"|'''Template'''
 +
| align="center" style="background:#f0f0f0;"|'''F Primer'''
 +
| align="center" style="background:#f0f0f0;"|'''R Primer'''
 +
| align="center" style="background:#f0f0f0;"|'''Annealing T'''
 +
| align="center" style="background:#f0f0f0;"|'''Expected length'''
 +
|-
 +
| LuxI||LuxI sender const 8/31/13 179ng||P063||P064||23||654
 +
|}

Revision as of 18:07, 12 May 2014

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mm/dd/yyyy

Ran PCR products from 5/6/14 on a gel. Only 6, RhlI worked.

Re-ran other PCRs, annealing temp 40, 4min ext

Number Template F Primer R Primer Annealing T Expected length
1pTet-mCh 2/14/14 74ngP127P097533148
2LuxI sender const 8/31/13 179ngP063P06423654
3K084007 3/10/13 4-2-1P069P07032645
4K084007 3/10/13 4-2-2P069P07032645
5RBS-LasI 3/8/13 31ngP069P07032645


Ran a second one of the backbone with annealing temp 50, ext time 4 min

Label Template F Primer R Primer Annealing T Expected length
mCh senderpTet-mCh 2/14/14 74ngP127P097533148



Ran on gel, only 5, LasI worked.

Ran a third, annealing temp 30

Label Template F Primer R Primer Annealing T Expected length
LuxILuxI sender const 8/31/13 179ngP063P06423654



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