Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28: Difference between revisions

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| F2620||P113||P114||51||817||||LuxR
| F2620||P113||P114||51||817||||LuxR
|-
|-
| K092900+eCFP||P117||P118||67||765||||RhlR
| K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
|-
| K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
|-
| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
|-
|-
| EsaR synthesized||P121||P122||50||758||||EsaR
| EsaR synthesized||P121||P122||50||758||||EsaR

Revision as of 12:01, 29 April 2014

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mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Template F primer R primer Tm Expected length Expected incorrect length Product
Receiver int P123 P112 49 2203 2430 Rec backbone
Receiver int P119 P107 48 1051 pLux
Receiver int P125 P107 48 1051 pRhl
Receiver int P126 P107 48 1051 pEsa
F2620 P113 P114 51 817 LuxR
K092900+eCFP (70ul/ng) P117 P118 67 765 RhlR
K092900+eCFP (74ng/ul) P117 P118 67 765 RhlR
K092900 (96ng/ul) P117 P118 67 765 RhlR
EsaR synthesized P121 P122 50 758 EsaR


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