Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/18: Difference between revisions

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(Autocreate 2014/04/18 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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Sequencing results: <br>
Reverse primer didn't give results, turns out I removed the binding site during cloning. <br>
Forward primer showed only backbone. Unsure what this means because the band size on the gel indicates the insert is there. Two possibilities: Backbone self-ligated or I moved the sequencing primer binding site to a new location.





Revision as of 11:29, 22 April 2014

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mm/dd/yyyy

Sequencing results:
Reverse primer didn't give results, turns out I removed the binding site during cloning.
Forward primer showed only backbone. Unsure what this means because the band size on the gel indicates the insert is there. Two possibilities: Backbone self-ligated or I moved the sequencing primer binding site to a new location.