Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/08: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) (Autocreate 2014/04/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
Checked second PCR reaction 3 on gel, looks like it's there. | |||
<br> | |||
Purify second reaction 3, DpnI digested 20ul reactions 1, 2, 4, 5. Heat inactivated. Cleaned using zymo DNA clean and concentration-5 kit. Added 100ul of DNA binding buffer to DpnI reactions, 125ul DNA binding buffer to 25ul of PCR reaction 3. (don't need to digest Reaction 3 because it wasn't amplifying a vector) | |||
Revision as of 11:35, 22 April 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
mm/dd/yyyyChecked second PCR reaction 3 on gel, looks like it's there.
|