Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/14: Difference between revisions

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==02/14/2014==
==02/14/2014==
Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders.  
Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders. <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| pTet-B0034-mCh-T-T in pSB1A3 5-1||0.108||0.056||1.927||108.225
|-
| pTet-B0034-mCh-T-T in pSB1A3 6-2||0.077||0.039||1.986||76.508
|-
| pTet-B0034-mCh-T-T in pSB1A3 6-1||0.074||0.036||2.058||74.252
|-
| pTet-B0034-mCh-T-T in pSB1A3 5-2||0.102||0.05||2.021||101.873
|-
|
|}
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<br><br>
Prepare K12 and DH5αT competent cells.
Prepare K12 and DH5αT competent cells.

Revision as of 14:16, 14 February 2014

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02/14/2014

Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders.

Sample Read# 260 280 260/280 ng/µL
pTet-B0034-mCh-T-T in pSB1A3 5-1 0.108 0.056 1.927 108.225
pTet-B0034-mCh-T-T in pSB1A3 6-2 0.077 0.039 1.986 76.508
pTet-B0034-mCh-T-T in pSB1A3 6-1 0.074 0.036 2.058 74.252
pTet-B0034-mCh-T-T in pSB1A3 5-2 0.102 0.05 2.021 101.873



Prepare K12 and DH5αT competent cells.



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