Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/28: Difference between revisions
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Rene M Davis (talk | contribs) |
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PCR ''need to edit this to include the next lane. lane 1 is P087/P088 and lane 2 is P089/P090, both I13522'' [[image:13.10.28_PCR_cropped.png|PCR]] | PCR ''need to edit this to include the next lane. lane 1 is P087/P088 and lane 2 is P089/P090, both I13522'' [[image:13.10.28_PCR_cropped.png|PCR]] | ||
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Ran another PCR reaction to text which primer wasn't working for the sender backbone. Used the two sender backbone primers, P089 and P090 with the matching F or R primer from the pLux vectors primers which were shown to work. Rxn 3 is positive control. | Ran another PCR reaction to text which primer wasn't working for the sender backbone. Used the two sender backbone primers, P089 and P090 with the matching F or R primer from the pLux vectors primers which were shown to work. Rxn 3 is positive control.<br> | ||
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:15ul reaction | :15ul reaction | ||
Revision as of 15:23, 15 November 2013
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mm/dd/yyyyMiniprepped J04450 that I started growing on Saturday. They were in the incubator for a long time and were super red.
Gel of cut J04450 PCR need to edit this to include the next lane. lane 1 is P087/P088 and lane 2 is P089/P090, both I13522
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