Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/28: Difference between revisions
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Rene M Davis (talk | contribs) |
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'''Miniprep'''<br> | '''Miniprep'''<br> | ||
{| {{table}} | |||
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|260 ||0.034 | |||
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|280 ||0.021 | |||
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|260/280|| 1.631 | |||
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|ng/µL ||33.893 | |||
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|} | |||
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'''Restriction digest'''<br> | '''Restriction digest'''<br> | ||
cut 15ul of 33ng/ul, so about 0.5ug of plasmid. Cut with E/X in a 20ul rxn for 25 minutes at 37°C<br> | cut 15ul of 33ng/ul, so about 0.5ug of plasmid. Cut with E/X in a 20ul rxn for 25 minutes at 37°C<br> |
Revision as of 15:30, 15 November 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||
mm/dd/yyyyMiniprepped J04450 that I started growing on Saturday. They were in the incubator for a long time and were super red.
Restriction digest Gel of cut J04450
PCR need to edit this to include the next lane. lane 1 is P087/P088 and lane 2 is P089/P090, both I13522
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