Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/25: Difference between revisions
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Rene M Davis (talk | contribs) |
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Because the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid<br> | Because the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid<br> | ||
[[image:13.10.25_cropped.png||J06702 digest]] | [[image:13.10.25_cropped.png||J06702 digest]] | ||
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The band should be 3024bp but it's closer to 4kb. I don't know what happened. I will try the ligation and then sequence. I'll also send the original miniprep off for sequencing. | |||
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Revision as of 17:27, 25 October 2013
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mm/dd/yyyyBecause the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid | |
