Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/23

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(Autocreate 2013/10/23 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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miniprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.<br>
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{| {{table}}
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|-
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|plasmid||260||280||260/280||concentration
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|-
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|J06702||0.247|| 0.128 ||1.93 ||247.2
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|-
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|ligation||0.071|| 0.035 ||2.006||70.9
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|-
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|}
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<br>
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Digested J06702 with
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:E only for 10 minutes
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:X only for 10 minutes
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:E and X for 10 minutes
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:E only for 5, then add X for 5 more minutes
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<br>
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Digested ligation with
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:P only
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:E/P
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<br>
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Ran on gel <br>
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13.10.23_Pre_ext.jpg‎
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<br>
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Extracted the bottom bands from the two J06702 cut with E/X <br>
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13.10.23_Post_ext.jpg‎
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<br>
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Zymo gel recover kit
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<br>
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{| {{table}}
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|-
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|plasmid||260||280||260/280||concentration
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|-
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|J06702 3||0.017 ||0.011 ||1.468|| 16.729
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|-
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|J06702 4||0.008 ||0.005|| 1.583||7.782
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|-
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|}

Revision as of 15:36, 24 October 2013

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mm/dd/yyyy

miniprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.

plasmid260280260/280concentration
J067020.247 0.128 1.93 247.2
ligation0.071 0.035 2.00670.9


Digested J06702 with

E only for 10 minutes
X only for 10 minutes
E and X for 10 minutes
E only for 5, then add X for 5 more minutes


Digested ligation with

P only
E/P


Ran on gel

13.10.23_Pre_ext.jpg‎


Extracted the bottom bands from the two J06702 cut with E/X

13.10.23_Post_ext.jpg‎


Zymo gel recover kit

plasmid260280260/280concentration
J06702 30.017 0.011 1.468 16.729
J06702 40.008 0.005 1.5837.782



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