Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/18: Difference between revisions

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(Autocreate 2013/10/18 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
Retry ligation with gel extracted J06702
<br>
Backbone: J06702 E/X: 44 ng/ul<br>
:Cut, gel purified
Insert: Gblck pLac-RBS E/S: 2.6ng/ul
:Cut, column purified<br>
"did a bunch of calculations, only actually tried 3:1 and bb ctrl''<br>
{| {{table}}
|-
| ||1:1||2:1||3:1||Bb ctrl
|-
|insert||0.5||1.0||1.5||0
|-
|vector||1.13||1.13||1.13||1.13
|-
|2x Roche ligation buffer||5||5||5||5
|-
|T4 ligase||1||1||1||1
|-
|H20||2.37||1.87||1.37||2.87
|-
|}
<br>
ligations ran for 25min at RT
<br>
Incubate with 40ul DH5αT for 15 min on ice




Revision as of 18:10, 21 October 2013

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mm/dd/yyyy

Retry ligation with gel extracted J06702
Backbone: J06702 E/X: 44 ng/ul

Cut, gel purified

Insert: Gblck pLac-RBS E/S: 2.6ng/ul

Cut, column purified

"did a bunch of calculations, only actually tried 3:1 and bb ctrl

1:1 2:1 3:1 Bb ctrl
insert 0.5 1.0 1.5 0
vector 1.13 1.13 1.13 1.13
2x Roche ligation buffer 5 5 5 5
T4 ligase 1 1 1 1
H20 2.37 1.87 1.37 2.87


ligations ran for 25min at RT
Incubate with 40ul DH5αT for 15 min on ice



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