Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/17: Difference between revisions
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'''12PM''' | '''12PM''' | ||
Pulled RhlI out at 12pm. pH looks between 7 and 8 (paper strip) | Pulled RhlI out at 12pm. pH looks between 7 and 8 (paper strip) |
Revision as of 16:15, 2 December 2013
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09/17/101312PM Pulled RhlI out at 12pm. pH looks between 7 and 8 (paper strip)
Added 1mL ethyl acetate to LB
Transfer 1mL of the top layer to 1.5mL tube
Let sit for 15-30 seconds
Transfer top layer to new 15mL conical tube
Store at -20°C
Tranformations into DH5αT and BL21
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