Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/08

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(mm/dd/yyyy)
Current revision (15:07, 12 September 2013) (view source)
(mm/dd/yyyy)
 
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{| {{table}}
{| {{table}}
|-
|-
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|Number||Template||Primer 1||Primer 2||Expected size
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|Number||Template||Primer 1||Primer 2||Expected size||Worked?
|-
|-
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|1||I13522 3||P060||P062||3136
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|1||I13522 3||P060||P062||3136|| No
|-
|-
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|2||K084007 12.9||P069||P070||645
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|2||K084007 12.9||P069||P070||645|| Yes
|-
|-
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|3||K084007 18.3||P069||P070||645
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|3||K084007 18.3||P069||P070||645|| No
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|-
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|4||K092900-E0420 80.5||P077||P078||765
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|4||K092900-E0420 80.5||P077||P078||765||Probably not
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|-
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|5||Part B1||P081||P082||980
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|5||Part B1||P081||P082||980|| No
|-
|-
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|6||Part B2||P081||P082||980
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|6||Part B2||P081||P082||980||No
|-
|-
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|7||Part B3||P081||P082||980
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|7||Part B3||P081||P082||980||No
|-
|-
|}
|}
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 +
[[image:13.09.09_GG_PCR_edited.png|600px|GG PCR round 2, not the ugliest gel I've run]]
 +

Current revision

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mm/dd/yyyy

Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)

NumberTemplatePrimer 1Primer 2Expected sizeWorked?
1I13522 3P060P0623136 No
2K084007 12.9P069P070645 Yes
3K084007 18.3P069P070645 No
4K092900-E0420 80.5P077P078765Probably not
5Part B1P081P082980 No
6Part B2P081P082980No
7Part B3P081P082980No

GG PCR round 2, not the ugliest gel I've run



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