Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/08: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==mm/dd/yyyy==
==mm/dd/yyyy==
Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)
Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)
{| {{table}}
|-
|Number||Template||Primer 1||Primer 2||Expected size||Worked?
|-
|1||I13522 3||P060||P062||3136|| No
|-
|2||K084007 12.9||P069||P070||645|| Yes
|-
|3||K084007 18.3||P069||P070||645|| No
|-
|4||K092900-E0420 80.5||P077||P078||765||Probably not
|-
|5||Part B1||P081||P082||980|| No
|-
|6||Part B2||P081||P082||980||No
|-
|7||Part B3||P081||P082||980||No
|-
|}
[[image:13.09.09_GG_PCR_edited.png|600px|GG PCR round 2, not the ugliest gel I've run]]




Latest revision as of 23:17, 26 September 2017

Project name Main project page
Previous entry      Next entry

mm/dd/yyyy

Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)

Number Template Primer 1 Primer 2 Expected size Worked?
1 I13522 3 P060 P062 3136 No
2 K084007 12.9 P069 P070 645 Yes
3 K084007 18.3 P069 P070 645 No
4 K092900-E0420 80.5 P077 P078 765 Probably not
5 Part B1 P081 P082 980 No
6 Part B2 P081 P082 980 No
7 Part B3 P081 P082 980 No

GG PCR round 2, not the ugliest gel I've run



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