Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/06

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09/06/2013

PCR for Golden Gate assemblies
Decided to redo everything.

NumberTemplatePrimer 1Primer 2Expected size
1I13522 73.4P060P0623136
2I13522 73.4PxP0843136
3I13522 73.4PyPz90
4I13522 73.4P060P0623136
5I13522 2PxP0843136
6I13522 2PyPz90
7I13522 73.4P060P0623136
8I13522 3PxP0843136
9I13522 3PyPz90
10K145254 4P063P064679
11K145254 7/24/13P063P064679
12F2620+GFP 7/24/13P065P066817
13F2620+GFP 7/24/13P067P06856
14K084007 7/24/13P069P070645
15K082035 36P075P076703
16K092900-E0420 80.5P077P078765
17K092900-E0420 80.5P079P08056
18Part B1P081P082980
19Part B2P081P082980
20Part B3P081P082980
21PSB1C3 (1)P083P0843000
22PSB1C3 (2)P083P0843000
23I13522 2P060P0623136
24I13522 3P060P0623136




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