Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/19

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Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br>
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br>
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.  
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.  
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Revision as of 19:07, 19 July 2013

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07/19/2013

Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
I03522 or whatever has colonies. Picked two.
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb.
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.

PartSizeMiniprep yield 1Miniprep yield 2
K092900+cyan~2900
F2620+GFP~2000
K082035 68
K084007835


Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors.



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