Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/19: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
||
Line 25: | Line 25: | ||
|} | |} | ||
<br> | <br> | ||
Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors. | Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors. Ran on a gel<br> | ||
{| {{table}} | |||
|- | |||
|No. || Name||Length||Gel check | |||
|- | |||
|1||LuxR-GFP Receiver|| 1900|| + | |||
|- | |||
|2||K092900 + E0240||2200||- | |||
|- | |||
|3 ||C0061 - 1||643||couldn't tell | |||
|- | |||
|4||C0061 - 2||643||couldn't tell | |||
|- | |||
|5||C0062||781||couldn't tell | |||
|- | |||
|6||F2620 (81ng/ul)||1061||+ | |||
|- | |||
|7||F2620 (52ng/ul)||1061||+ | |||
|- | |||
|8||K091134||2048||- | |||
|- | |||
|9||K092900||2102||_ | |||
|- | |||
|10||K145254||862||+ | |||
|- | |||
|11||K082035||884||+ | |||
|- | |||
|12||I0466||942||+ | |||
|- | |||
|13||K084007||835||+ | |||
|- | |||
|14||RBS-LasI||600||+ | |||
|- | |||
|15||messed up||?||- | |||
|- | |||
|16||K092900-cyan||2900||- | |||
|- | |||
|17||F2620-GFP||2000||- | |||
|- | |||
|18||K084007||835||+ | |||
|- | |||
|19||RBS-LuxI||798||not sure | |||
|- | |||
|20||J06702 56ng/ul||869||+ | |||
|- | |||
|} | |||
<br> | |||
Picked one colony from each of the positive ones and K091134 because I don't have another LasR option.<br> | |||
Revision as of 20:48, 21 July 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
07/19/2013Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
|