Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/19: Difference between revisions

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(Autocreate 2013/07/19 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==07/19/2013==
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Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case. <br>
I03522 or whatever has colonies. Picked two. <br>
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br>
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.
{|table|}
|-
|Part||Size||Miniprep yield 1||Miniprep yield 2
|-
|K092900+cyan||~2900||
|-
|F2620+GFP||~2000||
|-
|K082035|| || 6||8
|-
|K084007||835||
|-
|}
<br>
Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors.  




Revision as of 17:06, 19 July 2013

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07/19/2013

Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
I03522 or whatever has colonies. Picked two.
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb.
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.

Part Size Miniprep yield 1 Miniprep yield 2
K092900+cyan ~2900
F2620+GFP ~2000
K082035 6 8
K084007 835


Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors.



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