Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/29

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(Entry title)
Line 15: Line 15:
|LuxI||LuxR-GFP
|LuxI||LuxR-GFP
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|-
-
|LasI|| ||
+
|LasI||  
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|-
|RhlI|| RhlR-GFP (maybe)
|RhlI|| RhlR-GFP (maybe)
|-
|-
-
|TraI || ||
+
|TraI ||  
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|}
|}

Revision as of 13:00, 29 May 2013

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Entry title

Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.

Senders (6ml) Receivers (2ml)
LuxILuxR-GFP
LasI
RhlI RhlR-GFP (maybe)
TraI
Controls
Positive (Kind of)GFP only (insert part #)
Negative sender TraR
Negative receiver none



Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.


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