Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/29: Difference between revisions

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(Autocreate 2013/05/29 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==Entry title==
==Entry title==
* Insert content here...
Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.


{| {{table}}
|-
|Senders (6ml) || Receivers (2ml)
|-
|LuxI||LuxR-GFP
|-
|LasI|| ||
|-
|RhlI|| RhlR-GFP (maybe)
|-
|TraI || ||
|-
|}
{| {{table}}
|-
|Controls
|-
|Positive (Kind of)||GFP only (insert part #)
|-
|Negative sender || TraR
|-
|Negative receiver || none
|-
|}
<br> <br>
Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.


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Revision as of 11:00, 29 May 2013

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Entry title

Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.

Senders (6ml) Receivers (2ml)
LuxI LuxR-GFP
LasI
RhlI RhlR-GFP (maybe)
TraI
Controls
Positive (Kind of) GFP only (insert part #)
Negative sender TraR
Negative receiver none



Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.