Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/29

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(Autocreate 2013/05/29 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
(Entry title)
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==Entry title==
==Entry title==
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* Insert content here...
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Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.
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{| {{table}}
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|-
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|Senders (6ml) || Receivers (2ml)
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|-
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|LuxI||LuxR-GFP
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|-
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|LasI|| ||
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|-
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|RhlI|| RhlR-GFP (maybe)
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|-
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|TraI || ||
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|-
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|}
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{| {{table}}
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|-
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|Controls
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|-
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|Positive (Kind of)||GFP only (insert part #)
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|-
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|Negative sender || TraR
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|-
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|Negative receiver || none
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|-
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|}
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<br> <br>
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Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.
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Revision as of 14:00, 29 May 2013

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Entry title

Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.

Senders (6ml) Receivers (2ml)
LuxILuxR-GFP
LasI
RhlI RhlR-GFP (maybe)
TraI
Controls
Positive (Kind of)GFP only (insert part #)
Negative sender TraR
Negative receiver none



Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.


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