Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/14: Difference between revisions

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(Autocreate 2013/03/14 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==Entry title==
==Entry title==
* Insert content here...
picked a colony from the K092900+E0420 ligation plate, grew in 3ml LB for 10 hours
<br>
Dave thinks the GC data from hexane extraction might be ok but the standards are too high a concentration to be relevant to my sample concentrations. I looked at the data with Becky and Shawn's help and I couldn't find the peaks for my AHLs. I added 1ul of each AHL to 500ul of hexane or ethyl acetate to see if I can see the AHL peak with ethyl acetate. After I added the AHL to hexane, I noticed the droplet just sat on the bottom. I didn't mix my standards last time which might explain why I couldn't find my AHL peaks. I vortexed them this time.
<br>
minipreps:
K092900+E0420 ligation
 
found an E0420 round bottom tube in the incubator, not sure when it was from by I miniprepped it anyway
 
miniprep concentrations from today and some previous days:
 
{| {{table}}
|-
|Part||260||280||260/280||ng/ul
|-
|K092900m.p. 3/10/13 || 0.04||0.022||1.85||40.299
|-
|K092900+E0420 lig mp 3/13/13||0.081||0.042||1.902||80.549
|-
|E0420 mp||0.1||0.054||1.858||100.337
|-
|}
 
cut the two minipreps above and 5ul of old K092900 (from 3/10) and E0420 (from 3/3) all with X&P
 




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Entry title

picked a colony from the K092900+E0420 ligation plate, grew in 3ml LB for 10 hours
Dave thinks the GC data from hexane extraction might be ok but the standards are too high a concentration to be relevant to my sample concentrations. I looked at the data with Becky and Shawn's help and I couldn't find the peaks for my AHLs. I added 1ul of each AHL to 500ul of hexane or ethyl acetate to see if I can see the AHL peak with ethyl acetate. After I added the AHL to hexane, I noticed the droplet just sat on the bottom. I didn't mix my standards last time which might explain why I couldn't find my AHL peaks. I vortexed them this time.
minipreps: K092900+E0420 ligation

found an E0420 round bottom tube in the incubator, not sure when it was from by I miniprepped it anyway

miniprep concentrations from today and some previous days:

Part 260 280 260/280 ng/ul
K092900m.p. 3/10/13 0.04 0.022 1.85 40.299
K092900+E0420 lig mp 3/13/13 0.081 0.042 1.902 80.549
E0420 mp 0.1 0.054 1.858 100.337

cut the two minipreps above and 5ul of old K092900 (from 3/10) and E0420 (from 3/3) all with X&P



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