Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/14

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(Autocreate 2013/03/14 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (16:59, 28 May 2013) (view source)
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==Entry title==
==Entry title==
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picked a colony from the K092900+E0420 ligation plate, grew in 3ml LB for 10 hours
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<br>
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Dave thinks the GC data from hexane extraction might be ok but the standards are too high a concentration to be relevant to my sample concentrations. I looked at the data with Becky and Shawn's help and I couldn't find the peaks for my AHLs. I added 1ul of each AHL to 500ul of hexane or ethyl acetate to see if I can see the AHL peak with ethyl acetate. After I added the AHL to hexane, I noticed the droplet just sat on the bottom. I didn't mix my standards last time which might explain why I couldn't find my AHL peaks. I vortexed them this time.
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<br>
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minipreps:
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K092900+E0420 ligation
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found an E0420 round bottom tube in the incubator, not sure when it was from by I miniprepped it anyway
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miniprep concentrations from today and some previous days:
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{| {{table}}
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|-
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|Part||260||280||260/280||ng/ul
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|-
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|K092900m.p. 3/10/13 || 0.04||0.022||1.85||40.299
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|-
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|K092900+E0420 lig mp 3/13/13||0.081||0.042||1.902||80.549
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|-
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|E0420 mp||0.1||0.054||1.858||100.337
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|-
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|}
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cut the two minipreps above and 5ul of old K092900 (from 3/10) and E0420 (from 3/3) all with X&P
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Entry title

picked a colony from the K092900+E0420 ligation plate, grew in 3ml LB for 10 hours
Dave thinks the GC data from hexane extraction might be ok but the standards are too high a concentration to be relevant to my sample concentrations. I looked at the data with Becky and Shawn's help and I couldn't find the peaks for my AHLs. I added 1ul of each AHL to 500ul of hexane or ethyl acetate to see if I can see the AHL peak with ethyl acetate. After I added the AHL to hexane, I noticed the droplet just sat on the bottom. I didn't mix my standards last time which might explain why I couldn't find my AHL peaks. I vortexed them this time.
minipreps: K092900+E0420 ligation

found an E0420 round bottom tube in the incubator, not sure when it was from by I miniprepped it anyway

miniprep concentrations from today and some previous days:

Part260280260/280ng/ul
K092900m.p. 3/10/13 0.040.0221.8540.299
K092900+E0420 lig mp 3/13/130.0810.0421.90280.549
E0420 mp0.10.0541.858100.337

cut the two minipreps above and 5ul of old K092900 (from 3/10) and E0420 (from 3/3) all with X&P



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