Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07

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(Entry title)
(Entry title)
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transformed RBS+LuxI and RBS+LasI
transformed RBS+LuxI and RBS+LasI
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 +
{| {{table}}
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|-
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|Part||260||280||260/280||ng/ul
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|-
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|K079021||0.131||0.07||1.836||130.892
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|-
 +
|I13522||0.125||0.068||125.239
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|-
 +
|K079020||0.086||0.046||1.855||85.913
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|-
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|J45200||0.081||0.045||1.81||80.868
 +
|-
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|J04450||0.071||0.038||1.859||70.782
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|-
 +
|}

Revision as of 16:34, 28 May 2013

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minipreps

streak

cut and extract and clone

pull out promoters + RBS from registry

gel extract and ligate and transform part 6


miniprep C0061 1 and 2 C0062 K081016 that haynes grew up, streaked on plate (she found by PCR) R0071 that haynes grew up, streaking on plate " "

cut K081016 and R0071 from above, ran on a gel, didn't work

Ernesto ran colony PCR on a bunch of mini preps and colonies

transformed RBS+LuxI and RBS+LasI

Part260280260/280ng/ul
K0790210.1310.071.836130.892
I135220.1250.068125.239
K0790200.0860.0461.85585.913
J452000.0810.0451.8180.868
J044500.0710.0381.85970.782



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