Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07

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==Entry title==
==Entry title==
minipreps
minipreps
 +
streak
streak
 +
cut and extract and clone
cut and extract and clone
 +
pull out promoters + RBS from registry
pull out promoters + RBS from registry
 +
gel extract and ligate and transform part 6
gel extract and ligate and transform part 6

Revision as of 15:31, 28 May 2013

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minipreps

streak

cut and extract and clone

pull out promoters + RBS from registry

gel extract and ligate and transform part 6


miniprep C0061 1 and 2 C0062 K081016 that haynes grew up, streaked on plate (she found by PCR) R0071 that haynes grew up, streaking on plate " "

cut K081016 and R0071 from above, ran on a gel, didn't work

Ernesto ran colony PCR on a bunch of mini preps and colonies

transformed RBS+LuxI and RBS+LasI



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