Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/06: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/06 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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==Entry title== | ==Entry title== | ||
picked C0061 and C0062 and banana and RFP and GFP colonies for Ernesto to prep | |||
decided that the multiple bands on the gel are not what i want because the sizes don't make sense. decided 6 did work | |||
purified 6 PCR product using zymo clean and concentrator | |||
got good yield (100ng/ul) | |||
{| {{Table}} | |||
|- | |||
|Part||260||280||260/280||ng/ul | |||
|- | |||
|6 PCR purification||0.1||0.054||1.85||100.274 | |||
|- | |||
|} | |||
cut and ran on a gel | |||
cut piece out, put at -20 | |||
Revision as of 13:24, 28 May 2013
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Entry titlepicked C0061 and C0062 and banana and RFP and GFP colonies for Ernesto to prep decided that the multiple bands on the gel are not what i want because the sizes don't make sense. decided 6 did work purified 6 PCR product using zymo clean and concentrator got good yield (100ng/ul)
cut and ran on a gel cut piece out, put at -20
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