Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01: Difference between revisions
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Rene M Davis (talk | contribs) |
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{| {{table}} | {| {{table}} | ||
|- | |- | ||
| Part number || Description || Part size (bp) || Backbone || Colonies | | Number||Part number || Description || Part size (bp) || Backbone || Colonies | ||
|- | |- | ||
| || || || || 1 || 2 || 3 || 4 || 5 | | || || || || 1 || 2 || 3 || 4 || 5 | ||
|- | |- | ||
| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0 | | 1||F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0 | ||
|- | |- | ||
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X | | 2||K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X | ||
|- | |- | ||
| R0071 || RhlR-activated promoter || 53 || pSB1A2 || X || | | 3||R0071 || RhlR-activated promoter || 53 || pSB1A2 || X || | ||
|- | |- | ||
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X | |4|| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X | ||
|- | |- | ||
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X | |5|| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X | ||
|- | |- | ||
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X | | 6||K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X | ||
|} | |} | ||
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{| {{table}} | {| {{table}} | ||
|- | |- | ||
| Part number || Description || Part size (bp) || Backbone | | Number ||Part number || Description || Part size (bp) || Backbone | ||
|- | |- | ||
|K145254 ||Plac+RBS+LuxI+Stop+Stop|| 862 ||pSB1A2 | |7||K145254 ||Plac+RBS+LuxI+Stop+Stop|| 862 ||pSB1A2 | ||
|- | |- | ||
|K091134 || Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S ||2048 ||pSB1AK3 | |8||K091134 || Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S ||2048 ||pSB1AK3 | ||
|- | |- | ||
|K092100 || Pconst+RBS+RhlR+S+S|| 930 || pSB1A2 | |9||K092100 || Pconst+RBS+RhlR+S+S|| 930 || pSB1A2 | ||
|- | |- | ||
|K092900 || Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac ||2102 || pSB1A2 | |10||K092900 || Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac ||2102 || pSB1A2 | ||
|- | |- | ||
|E0240 || RBS+GFP+S+S ||876 || pSB1A2 | |11||E0240 || RBS+GFP+S+S ||876 || pSB1A2 | ||
|} | |} | ||
Revision as of 16:15, 28 May 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Entry titleMiniprepped the colonies grown up yesterday
Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.
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