Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01

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| Part number || Description || Part size (bp) || Backbone  
| Part number || Description || Part size (bp) || Backbone  
|-
|-
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| K145254 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2  
+
|K145254 ||Plac+RBS+LuxI+Stop+Stop|| 862 ||pSB1A2
|-
|-
-
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2
+
|K091134 || Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S ||2048 ||pSB1AK3
|-
|-
-
| R0071 || RhlR-activated promoter || 53 || pSB1A2  
+
|K092100 || Pconst+RBS+RhlR+S+S|| 930 || pSB1A2
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|-
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| K084007 || LacI-RBS-LasI || 835 || pSB1AK3
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|K092900 || Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac ||2102 || pSB1A2
|-
|-
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| K081016 || RBS-lasI-Stop || 735 || pSB1AK3
+
|E0240 || RBS+GFP+S+S ||876 || pSB1A2
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|-
+
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| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3
+
|}
|}
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Miniprepped the colonies grown up yesterday

Part number Description Part size (bp) Backbone Colonies
1 2 3 4 5
F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2 0 0 X X 0
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2 X X
R0071 RhlR-activated promoter 53 pSB1A2 X
K084007 LacI-RBS-LasI 835 pSB1AK3 X X
K081016 RBS-lasI-Stop 735 pSB1AK3 0 X
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3 X 0 X

Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.


Pulled more parts out of the registry

Part number Description Part size (bp) Backbone
K145254 Plac+RBS+LuxI+Stop+Stop 862 pSB1A2
K091134 Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S 2048 pSB1AK3
K092100 Pconst+RBS+RhlR+S+S 930 pSB1A2
K092900 Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac 2102 pSB1A2
E0240 RBS+GFP+S+S 876 pSB1A2



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