Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01
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==Entry title== | ==Entry title== | ||
| - | |||
| + | Miniprepped the colonies grown up yesterday | ||
| + | {| {{table}} | ||
| + | |- | ||
| + | | Part number || Description || Part size (bp) || Backbone || Colonies | ||
| + | |- | ||
| + | | || || || || 1 || 2 || 3 || 4 || 5 | ||
| + | |- | ||
| + | | F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0 | ||
| + | |- | ||
| + | | K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X | ||
| + | |- | ||
| + | | R0071 || RhlR-activated promoter || 53 || pSB1A2 || X || | ||
| + | |- | ||
| + | | K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X | ||
| + | |- | ||
| + | | K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X | ||
| + | |- | ||
| + | | K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X | ||
| + | |} | ||
| + | |||
| + | Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea. | ||
| + | |||
| + | <br> | ||
| + | Pulled more parts out of the registry | ||
| + | |||
| + | {| {{table}} | ||
| + | |- | ||
| + | | Part number || Description || Part size (bp) || Backbone | ||
| + | |- | ||
| + | | K145254 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 | ||
| + | |- | ||
| + | | K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 | ||
| + | |- | ||
| + | | R0071 || RhlR-activated promoter || 53 || pSB1A2 | ||
| + | |- | ||
| + | | K084007 || LacI-RBS-LasI || 835 || pSB1AK3 | ||
| + | |- | ||
| + | | K081016 || RBS-lasI-Stop || 735 || pSB1AK3 | ||
| + | |- | ||
| + | | K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 | ||
| + | |} | ||
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Revision as of 16:59, 4 March 2013
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Entry titleMiniprepped the colonies grown up yesterday
Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.
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