Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01

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(Autocreate 2013/03/01 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
(Entry title)
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==Entry title==
==Entry title==
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* Insert content here...
 
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Miniprepped the colonies grown up yesterday
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{| {{table}}
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|-
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| Part number || Description || Part size (bp) || Backbone || Colonies
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|-
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| || || || || 1 || 2 || 3 || 4 || 5
 +
|-
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| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0
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|-
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| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X
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|-
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| R0071 || RhlR-activated promoter || 53 || pSB1A2 || X || 
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|-
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| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X
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|-
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| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X
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|-
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| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X
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|}
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Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.
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<br>
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Pulled more parts out of the registry
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 +
{| {{table}}
 +
|-
 +
| Part number || Description || Part size (bp) || Backbone
 +
|-
 +
| K145254 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2
 +
|-
 +
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2
 +
|-
 +
| R0071 || RhlR-activated promoter || 53 || pSB1A2
 +
|-
 +
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3
 +
|-
 +
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3
 +
|-
 +
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3
 +
|}
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Revision as of 15:59, 4 March 2013

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Entry title

Miniprepped the colonies grown up yesterday

Part number Description Part size (bp) Backbone Colonies
1 2 3 4 5
F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2 0 0 X X 0
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2 X X
R0071 RhlR-activated promoter 53 pSB1A2 X
K084007 LacI-RBS-LasI 835 pSB1AK3 X X
K081016 RBS-lasI-Stop 735 pSB1AK3 0 X
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3 X 0 X

Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.


Pulled more parts out of the registry

Part number Description Part size (bp) Backbone
K145254 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2
R0071 RhlR-activated promoter 53 pSB1A2
K084007 LacI-RBS-LasI 835 pSB1AK3
K081016 RBS-lasI-Stop 735 pSB1AK3
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3


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