Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/01 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
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==Entry title== | ==Entry title== | ||
Miniprepped the colonies grown up yesterday | |||
{| {{table}} | |||
|- | |||
| Part number || Description || Part size (bp) || Backbone || Colonies | |||
|- | |||
| || || || || 1 || 2 || 3 || 4 || 5 | |||
|- | |||
| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0 | |||
|- | |||
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X | |||
|- | |||
| R0071 || RhlR-activated promoter || 53 || pSB1A2 || X || | |||
|- | |||
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X | |||
|- | |||
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X | |||
|- | |||
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X | |||
|} | |||
Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea. | |||
<br> | |||
Pulled more parts out of the registry | |||
{| {{table}} | |||
|- | |||
| Part number || Description || Part size (bp) || Backbone | |||
|- | |||
| K145254 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 | |||
|- | |||
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 | |||
|- | |||
| R0071 || RhlR-activated promoter || 53 || pSB1A2 | |||
|- | |||
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 | |||
|- | |||
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 | |||
|- | |||
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 | |||
|} | |||
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Revision as of 13:59, 4 March 2013
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Entry titleMiniprepped the colonies grown up yesterday
Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.
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