Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/02/28

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(Autocreate 2013/02/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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==Entry title==
==Entry title==
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Did minipreps on the colonies picked 2013-02-27 (shook for 24 hours)<br>
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Many of them did not work. This chart shows which ones worked with an X and which ones did not grow with a 0:
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{| {{table}}
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|-
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| Part number || Description || Part size (bp) || Backbone || Colonies
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|-
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| || || || || 1 || 2
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|-
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| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0
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|-
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| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X
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|-
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| R0071 || RhlR-activated promoter || 53 || pSB1A2 || 0 || X
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|-
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| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X
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|-
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| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X
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|-
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| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0
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|}
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Picked more colonies and grew them in 3ml LB + amp
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Revision as of 16:52, 4 March 2013

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Entry title

Did minipreps on the colonies picked 2013-02-27 (shook for 24 hours)
Many of them did not work. This chart shows which ones worked with an X and which ones did not grow with a 0:


Part number Description Part size (bp) Backbone Colonies
1 2
F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2 0 0
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2 X X
R0071 RhlR-activated promoter 53 pSB1A2 0 X
K084007 LacI-RBS-LasI 835 pSB1AK3 X X
K081016 RBS-lasI-Stop 735 pSB1AK3 0 X
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3 X 0



Picked more colonies and grew them in 3ml LB + amp


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