Haynes Lab:Notebook/CRISPR Editing/2016/08/31: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/08/31 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
Rene M Davis (talk | contribs) |
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== | ==08/31/2016== | ||
Finished prepping sonicated chromatin from yesterday to test on a gel. Gel looked alirght, started ChIPs for | |||
:Gal4 +puro untreated | |||
:Luc14 g031 | |||
:Luc14 g034<br> | |||
Will do all the replicates on the same day to try to reduce error | |||
<br><br> | |||
31 | |||
:53 | |||
:55 | |||
:50 | |||
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new: 38 | |||
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34 | |||
:45 | |||
:51 | |||
:40 | |||
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new:64 | |||
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combined the old ones, will have plenty to do Gd34, which is the chromatin prep that didn't work yesterday. Perfect! | |||
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Gd 31 old won't have enoguh but I can combine it with the new chromatin prep and have plenty. | |||
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To double check that the chromatin is alright, I took 10ul of each to test on a gel. Started uncrosslinking at 5:20pm | |||
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Revision as of 17:50, 31 August 2016
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08/31/2016Finished prepping sonicated chromatin from yesterday to test on a gel. Gel looked alirght, started ChIPs for
Will do all the replicates on the same day to try to reduce error
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