Haynes Lab:Notebook/CRISPR Editing/2016/08/13: Difference between revisions
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== | ==08/13/2016== | ||
finished evaporating plasmids, got to high concentrations (31 = 1037ng/ul and 34=937ng/ul) and 260/280 ~1.9 | finished evaporating plasmids, got to high concentrations (31 = 1037ng/ul and 34=937ng/ul) and 260/280 ~1.9 | ||
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fixed two T-175 flasks of Gal4+puro untreated cells for H3K27me3 ChIP. trypsinized and did in 50mL conicals, 20mL of 1% formaldehyde | |||
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electroporated Luc14s and gal4+dox cells with g031 and 34, two 10cm plates per combo for 8 total plates. 20ug per transfection<br> | electroporated Luc14s and gal4+dox cells with g031 and 34, two 10cm plates per combo for 8 total plates. 20ug per transfection<br> | ||
prepped T-175 plated, resuspended to a final volume of 500ul | prepped T-175 plated, resuspended to a final volume of 500ul | ||
Revision as of 23:11, 13 August 2016
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08/13/2016finished evaporating plasmids, got to high concentrations (31 = 1037ng/ul and 34=937ng/ul) and 260/280 ~1.9
electroporated Luc14s and gal4+dox cells with g031 and 34, two 10cm plates per combo for 8 total plates. 20ug per transfection
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