Haynes Lab:Notebook/CRISPR Editing/2016/06/13: Difference between revisions

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<br>
<br>
try new off target site for FLAG antibody binding, located at the end of the luciferase transgene, just in case something weird is happening at the GAPDH site or with those primers<br>
try new off target site for FLAG antibody binding, located at the end of the luciferase transgene, just in case something weird is happening at the GAPDH site or with those primers<br>
 
<br>
master mix for P313/311<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''85'''
|-
| 2x SYBR mix||7.5||637.5
|-
| 750nmol F/R primer mix||3||255
|-
| PCR water||2.5||212.5
|-
| total||13||1105
|}
<br>
master mix for P157/158<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''11'''
|-
| 2x SYBR mix||7.5||82.5
|-
| 750nmol F/R primer mix||3||33
|-
| PCR water||2.5||27.5
|-
| total||13||143
|}
[[Image:16.06.13 qPCR plate 9 screen shot.png]]
[[Image:16.06.13 qPCR plate 9 screen shot.png]]


Revision as of 15:24, 13 June 2016

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06/13/2016

qPCR plate 9
all L and G 25 for H3K27me3 for the new g031/34 primer set P313/311
try new off target site for FLAG antibody binding, located at the end of the luciferase transgene, just in case something weird is happening at the GAPDH site or with those primers

master mix for P313/311

' 1 well 85
2x SYBR mix 7.5 637.5
750nmol F/R primer mix 3 255
PCR water 2.5 212.5
total 13 1105


master mix for P157/158

' 1 well 11
2x SYBR mix 7.5 82.5
750nmol F/R primer mix 3 33
PCR water 2.5 27.5
total 13 143


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