Haynes Lab:Notebook/CRISPR Editing/2016/06/06: Difference between revisions

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(Autocreate 2016/06/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==06/06/2016==
 
Cloned g032, 46, 54, and 55 into pX330_dCas9_EGFP
<br><br>
Re-annealed Luc14 g054 and 55 samples, redid surveyor assay, ran on bioanalyzer
<br><br>
'''ChIP qPCR plate 7'''<br>
tested g031/34 primer sets again, 1 to 2 dilutions of Luc14 sheared gDNA, 27ng/ul. 2ul into each reaction.
<br><br>
induced t-25 with dox, expanded most of the cells into t-75. will use for donor experiment and KAH228+g032 and/or 46.
<br>




Revision as of 00:08, 7 June 2016

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06/06/2016

Cloned g032, 46, 54, and 55 into pX330_dCas9_EGFP

Re-annealed Luc14 g054 and 55 samples, redid surveyor assay, ran on bioanalyzer

ChIP qPCR plate 7
tested g031/34 primer sets again, 1 to 2 dilutions of Luc14 sheared gDNA, 27ng/ul. 2ul into each reaction.

induced t-25 with dox, expanded most of the cells into t-75. will use for donor experiment and KAH228+g032 and/or 46.



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