Haynes Lab:Notebook/CRISPR Editing/2016/05/31: Difference between revisions

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[[Image:16.05.30 qPCR Plate 2 screen shot.png]]
[[Image:16.05.30 qPCR Plate 2 screen shot.png]]


<br><br>
'''Plate 3'''
<br>
Primer set 1 MM, 21 wells
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''24'''
|-
| 2x SYBR mix||7.5||180
|-
| 750nmol F/R primer mix||3||72
|-
| PCR water||2.5||60
|-
| total||13||312
|}
<br>
Primer set 2 MM, 57 wells
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''36'''
|-
| 2x SYBR mix||7.5||270
|-
| 750nmol F/R primer mix||3||108
|-
| PCR water||2.5||90
|-
| total||13||468
|}
<br>
[[Image:16.05.30 qPCR Plate 3 screen shot.png]]


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Revision as of 13:41, 31 May 2016

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05/31/2016

qPCR for ChIP
Plate 2
Dropping Primer set 3 because the input calls were around 39 so there's no chance we'd see any pulldown DNA. Josh gave me new primers against GAPDH promoter he's been using for his ChIPs. Will test those for one input set on this plate.

Also adding wells with high variability between technical replicates. All the Luc14 samples on this plate are re-runs.

Primer set 1 MM, 39 wells

' 1 well 42
2x SYBR mix 7.5 315
750nmol F/R primer mix 3 126
PCR water 2.5 105
total 13 546


Primer set 2 MM, 33 wells

' 1 well 36
2x SYBR mix 7.5 270
750nmol F/R primer mix 3 108
PCR water 2.5 90
total 13 468

Primer set 3 MM, 12 wells

' 1 well 14
2x SYBR mix 7.5 105
750nmol F/R primer mix 3 42
PCR water 2.5 35
total 13 182



Plate 3

Primer set 1 MM, 21 wells

' 1 well 24
2x SYBR mix 7.5 180
750nmol F/R primer mix 3 72
PCR water 2.5 60
total 13 312


Primer set 2 MM, 57 wells

' 1 well 36
2x SYBR mix 7.5 270
750nmol F/R primer mix 3 108
PCR water 2.5 90
total 13 468



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