Haynes Lab:Notebook/CRISPR Editing/2016/05/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:
'''qPCR for ChIP!'''<br>
'''qPCR for ChIP!'''<br>
Primer sets
Primer sets
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Primer set'''
| align="center" style="background:#f0f0f0;"|'''Target'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''amplicon length'''
|-
| 1||g025||||||
|-
| 2||g031, g034||P308||P160||202
|-
| 3||GAPDH||P203||P204||
|}
<br><br>
Make the dilution curves for all three primer sets<br>
Will use just Luc14 gDNA, maybe 10 dilution points in triplicate, will be 90
<br><br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Primer set'''
| align="center" style="background:#f0f0f0;"|'''Triplicate'''
|-
| 25 1 H||1||3
|-
| 25 2 H||1||3
|-
| 25 3 H||1||3
|-
| 25 1 I||1||3
|-
| 25 2 I||1||3
|-
| 25 3 I||1||3
|-
| 25 1 H||2||3
|-
| 25 2 H||2||3
|-
| 25 3 H||2||3
|-
| 25 1 I||2||3
|-
| 25 2 I||2||3
|-
| 25 3 I||2||3
|-
| 25 1 H||3||3
|-
| 25 2 H||3||3
|-
| 25 3 H||3||3
|-
| 25 1 I||3||3
|-
| 25 2 I||3||3
|-
| 25 3 I||3||3
|-
| ||||
|-
| 25 1 F||1||3
|-
| 25 2 F||1||3
|-
| 25 3 F||1||3
|-
| 25 1 F||3||3
|-
| 25 2 F||3||3
|-
| 25 3 F||3||3
|}
<br><br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Primer set'''
| align="center" style="background:#f0f0f0;"|'''Triplicate'''
|-
| 31 1 F||2||3
|-
| 31 2 F||2||3
|-
| 31 3 F||2||3
|-
| 31 1 I||2||3
|-
| 31 2 I||2||3
|-
| 31 3 I||2||3
|-
| 31 1 F||3||3
|-
| 31 2 F||3||3
|-
| 31 3 F||3||3
|-
| 31 1 I||3||3
|-
| 31 2 I||3||3
|-
| 31 3 I||3||3
|}
<br><br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Primer set'''
| align="center" style="background:#f0f0f0;"|'''Triplicate'''
|-
| 34 1 F||2||3
|-
| 34 2 F||2||3
|-
| 34 3 F||2||3
|-
| 34 1 I||2||3
|-
| 34 2 I||2||3
|-
| 34 3 I||2||3
|-
| 34 1 F||3||3
|-
| 34 2 F||3||3
|-
| 34 3 F||3||3
|-
| 34 1 I||3||3
|-
| 34 2 I||3||3
|-
| 34 3 I||3||3
|}
<br><br>
'''Redo nested PCR of the KAH228/g034 experiments'''<br>
Did it with the wrong primers the first time, need to redo with P149/176.
<br><br>
'''Redo nested PCR of the Luc14 g034 samples'''<br>
Did it with the wrong primers the first time, need to redo with P149/176. (need to double check with notebook to make sure I used 163/215).
<br><br>




Revision as of 15:50, 30 May 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

05/30/2016

qPCR for ChIP!
Primer sets

Primer set Target F primer R primer amplicon length
1 g025
2 g031, g034 P308 P160 202
3 GAPDH P203 P204



Make the dilution curves for all three primer sets
Will use just Luc14 gDNA, maybe 10 dilution points in triplicate, will be 90



Sample Primer set Triplicate
25 1 H 1 3
25 2 H 1 3
25 3 H 1 3
25 1 I 1 3
25 2 I 1 3
25 3 I 1 3
25 1 H 2 3
25 2 H 2 3
25 3 H 2 3
25 1 I 2 3
25 2 I 2 3
25 3 I 2 3
25 1 H 3 3
25 2 H 3 3
25 3 H 3 3
25 1 I 3 3
25 2 I 3 3
25 3 I 3 3
25 1 F 1 3
25 2 F 1 3
25 3 F 1 3
25 1 F 3 3
25 2 F 3 3
25 3 F 3 3



Sample Primer set Triplicate
31 1 F 2 3
31 2 F 2 3
31 3 F 2 3
31 1 I 2 3
31 2 I 2 3
31 3 I 2 3
31 1 F 3 3
31 2 F 3 3
31 3 F 3 3
31 1 I 3 3
31 2 I 3 3
31 3 I 3 3



Sample Primer set Triplicate
34 1 F 2 3
34 2 F 2 3
34 3 F 2 3
34 1 I 2 3
34 2 I 2 3
34 3 I 2 3
34 1 F 3 3
34 2 F 3 3
34 3 F 3 3
34 1 I 3 3
34 2 I 3 3
34 3 I 3 3



Redo nested PCR of the KAH228/g034 experiments
Did it with the wrong primers the first time, need to redo with P149/176.



Redo nested PCR of the Luc14 g034 samples
Did it with the wrong primers the first time, need to redo with P149/176. (need to double check with notebook to make sure I used 163/215).






Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2016/05/30&oldid=942074"