05/30/2016
qPCR for ChIP!
Primer sets
Primer set
|
Target
|
F primer
|
R primer
|
amplicon length
|
1 |
g025 |
P331 |
P329 |
205
|
2 |
g031, g034 |
P308 |
P160 |
202
|
3 |
GAPDH |
P203 |
P204 |
|
Plate 1
Luc14
- Reps 1-3
- H3K27me3, IgG, input
- Primer set 1, Primer set 2, Primer set 3
Primer master mix for primer sets 1, 2, and 3
'
|
1 wells
|
34
|
2x SYBR mix |
7.5 |
255
|
750nmol F/R primer mix |
3 |
102
|
PCR water |
2.5 |
85
|
total |
13 |
442
|
Plate 2
Gal4+dox
- Reps 1-3
- H3K27me3, IgG, input
- Primer set 1, Primer set 2, Primer set 3
Primer master mix for primer sets 1, 2, and 3
'
|
1 wells
|
34
|
2x SYBR mix |
7.5 |
255
|
750nmol F/R primer mix |
3 |
102
|
PCR water |
2.5 |
85
|
total |
13 |
442
|
Redo nested PCR of the KAH228/g034 experiments
Did it with the wrong primers the first time, need to redo with P149/176.
Redo nested PCR of the Luc14 g034 samples
Did it with the wrong primers the first time, need to redo with P149/176.
|