Haynes Lab:Notebook/CRISPR Editing/2016/05/24: Difference between revisions
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Rene M Davis (talk | contribs) |
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Added proteinase k to 25 sonicated chromatin. 62 for 2 hours | Added proteinase k to 25 sonicated chromatin. 62 for 2 hours | ||
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conc of sheared chromatin | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cells''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 50ug''' | |||
| align="center" style="background:#f0f0f0;"|'''ul dil buff''' | |||
|- | |||
| Luc14||25||1||2.272||1.32||1.721||2271.864||22.01||177.99 | |||
|- | |||
| Luc14||25||2||2.426||1.42||1.708||2426.165||20.61||179.39 | |||
|- | |||
| Luc14||25||3||2.022||1.17||1.728||2021.664||24.73||175.27 | |||
|- | |||
| Gal4||25||1||2.128||1.267||1.68||2127.688||23.50||176.50 | |||
|- | |||
| Gal4||25||2||1.305||0.795||1.643||1305.148||38.31||161.69 | |||
|- | |||
| Gal4||25||3||1.903||1.138||1.672||1902.941||26.28||173.72 | |||
|} | |||
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Revision as of 13:22, 24 May 2016
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05/24/2015Added RNase to g025 sonicated chromatin, 30min at 37
Can run surveyor on g025 and 31 today. Set up annealing reaction:
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