Haynes Lab:Notebook/CRISPR Editing/2016/05/24: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/05/24 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
Rene M Davis (talk | contribs) |
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== | ==05/24/2015== | ||
Added RNase to g025 sonicated chromatin, 30min at 37 | |||
<br><br> | |||
finally got enough DNA from PCR for g025 3. Means there's probably something wrong with my Phusion. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul hyb buff''' | |||
| align="center" style="background:#f0f0f0;"|'''ul H2O''' | |||
|- | |||
| Luc14||g025||3||0.034||0.019||1.809||33.885||11.80||1.50||1.70 | |||
|} | |||
Can run surveyor on g025 and 31 today. Set up annealing reaction: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul hyb buff''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
|- | |||
| Luc14||25||1||0.035||0.02||1.792||35.279||11.34||1.50||2.16 | |||
|- | |||
| Luc14||25||2||0.046||0.025||1.876||46.254||8.65||1.50||4.85 | |||
|- | |||
| Luc14||25||3||0.034||0.019||1.809||33.885||11.80||1.50||1.70 | |||
|- | |||
| |||||||||||||||||| | |||
|- | |||
| Luc14||31||1||0.07||0.037||1.9||69.982||5.72||1.50||7.78 | |||
|- | |||
| Luc14||31||2||0.087||0.046||1.885||86.915||4.60||1.50||8.90 | |||
|- | |||
| Luc14||31||3||0.035||0.019||1.868||35.484||11.27||1.50||2.23 | |||
|} | |||
<br><br> | |||
Added proteinase k to 25 sonicated chromatin. 62 for 2 hours | |||
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Revision as of 12:04, 24 May 2016
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05/24/2015Added RNase to g025 sonicated chromatin, 30min at 37
Can run surveyor on g025 and 31 today. Set up annealing reaction:
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