Haynes Lab:Notebook/CRISPR Editing/2016/05/22: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2016/05/22 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==03//2015==
==03//2015==
 
gel of P197 198 44, 46, 54, 55. All failed
<br><br>
gel of nPCR of g025 and 31. PCR pur of the ones that didn't fail
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul hyb buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Luc14||25||2||0.046||0.025||1.876||46.254||8.65||1.50||4.85
|-
| Luc14||31||1||0.07||0.037||1.9||69.982||5.72||1.50||7.78
|-
| Luc14||31||2||0.087||0.046||1.885||86.915||4.60||1.50||8.90
|-
| Luc14||31||3||0.035||0.019||1.868||35.484||11.27||1.50||2.23
|}
<br>
Re-run g025 rep 3, 32 cycles, more starting material
<br><br>
Re-run g044 and 46 P197/198




Revision as of 18:06, 22 May 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

03//2015

gel of P197 198 44, 46, 54, 55. All failed

gel of nPCR of g025 and 31. PCR pur of the ones that didn't fail

Cell type gRNA rep 260 280 260/280 ng/µL ul for 400ng ul hyb buff ul water
Luc14 25 2 0.046 0.025 1.876 46.254 8.65 1.50 4.85
Luc14 31 1 0.07 0.037 1.9 69.982 5.72 1.50 7.78
Luc14 31 2 0.087 0.046 1.885 86.915 4.60 1.50 8.90
Luc14 31 3 0.035 0.019 1.868 35.484 11.27 1.50 2.23


Re-run g025 rep 3, 32 cycles, more starting material

Re-run g044 and 46 P197/198



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2016/05/22&oldid=941707"