Haynes Lab:Notebook/CRISPR Editing/2016/02/27: Difference between revisions
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Rene M Davis (talk | contribs) |
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:0.3ul FseI | :0.3ul FseI | ||
:38ul H2O | :38ul H2O | ||
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Ran all cut backbones on gel, all look good! | |||
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Revision as of 13:06, 27 February 2016
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02/27/2016Finally found the FseI in the -80! Cut 1ug of pX330A_dCas9_1x4 vector from addgene and 80ng insert (gB009) with 1ul FseI for 1h at 37degC then deactivated at 65degC for 20min. Did three tubes of the backbone.
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