Haynes Lab:Notebook/CRISPR Editing/2016/02/24: Difference between revisions
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'''conclusion:''' background signal was really high, likely because the blocking buffer was less stringent than when KAH did hers with milk. can compare this image to the ponceau stain, see if the ratio of the stained bands is higher. should redo with much less secondary antibody, maybe dilute 1:3000 or more. | '''conclusion:''' background signal was really high, likely because the blocking buffer was less stringent than when KAH did hers with milk. can compare this image to the ponceau stain, see if the ratio of the stained bands is higher. should redo with much less secondary antibody, maybe dilute 1:3000 or more. | ||
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Cut 2ug plasmid with 0.5ul SgrAI in Cutsmart for 25 minutes. Heat inactivated, ran 0.5ul on gel<br> | |||
[[image:Screenshot 2016-03-12 20.18.45]] | |||
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Revision as of 20:21, 12 March 2016
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02/24/2016Western secondary antibody and imaging
STAINING HRP
set the membrane protein side down so it soaks in the bubble. drag the membrane until the bottom edge is just touching the bubble, then slowly lay the membrane across.
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