Haynes Lab:Notebook/CRISPR Editing/2016/02/23: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 8: Line 8:
==02/23/2016==
==02/23/2016==
'''Test qPCR primers for ChIP'''<br>
'''Test qPCR primers for ChIP'''<br>
Dilute purified DNA from ChIP 1:100<br>
Dilute Gal4-EED 1 sonicated and column purified DNA from ChIP 1:100<br>
Each reaction
Each reaction
:1ul diluted template
:1ul diluted template

Revision as of 08:46, 23 February 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

02/23/2016

Test qPCR primers for ChIP
Dilute Gal4-EED 1 sonicated and column purified DNA from ChIP 1:100
Each reaction

1ul diluted template
1ul F primer
1ul R primer
7ul water
10ul Taq 2x MM


Tubes 1-5

Tm 54

Tubes 6-10

Tm 47


Tube F primer R primer amplicon length Tm for taq
1 P313 P311 186 54
2 P309 P160 171 54
3 P309 P312 175 56
4 P308 P160 202 54
5 P308 P311 171 54
6 P314 P318 205 47
7 P316 P317 169 48
8 P315 P318 197 47
9 P316 P318 160 47
10 P316 P214 196 48



Cut MV13-KAH60
Test SgrAI

1ul SgrAI
1ul Fastdigest buffer


Test XbaI

1ul XbaI
1ul Cutsmart buffer

Incubate at 37 for 1 hour
Confirm on gel