Haynes Lab:Notebook/CRISPR Editing/2016/02/19: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/02/19 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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Collected the gRNA transfected Luc14 cells. Ran flow and froze the rest in lysis buffer from the gRNA extraction kit. | |||
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Extracted protein following this [https://benchling.com/hayneslab/f/8iVwOlNf-protein-and-enzyme-assays/prt-uT6b5OK6-mammalian-protein-extraction/edit protocol] from some Luc14 cells to do a western blot to verify the anti-H3K27me3 antibody. | |||
Revision as of 17:53, 22 February 2016
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02/19/2016Collected the gRNA transfected Luc14 cells. Ran flow and froze the rest in lysis buffer from the gRNA extraction kit.
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