Haynes Lab:Notebook/CRISPR Editing/2016/02/05: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/02/05 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
Rene M Davis (talk | contribs) |
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== | ==02/05/2016== | ||
Cass purified and ran sonicated DNA on gel <br> | |||
Lanes | |||
#1kb+ ladder | |||
#Gal4 EED 1, unsonicated | |||
#Gal4 EED 1, uncleaned and sonicated | |||
#Gal4 EED 1, cleaned and sonicated | |||
#Gal4 EED 2, unsonicated | |||
#Gal4 EED 2, uncleaned and sonicated | |||
#Gal4 EED 2, cleaned and sonicated | |||
#Luc14 1, unsonicated | |||
#Luc14 1, uncleaned and sonicated | |||
#Luc14 1, cleaned and sonicated | |||
#Luc14 2, unsonicated | |||
#Luc14 2, uncleaned and sonicated | |||
#Luc14 2, cleaned and sonicated | |||
#1kb+ ladder | |||
<br> | |||
Spec'd on nanodrop | |||
:Gal4 EED 1, cleaned and sonicated: 152.9 ng/uL | |||
:Gal4 EED 2, cleaned and sonicated: 204.7 ng/uL | |||
:Luc14 1, cleaned and sonicated: 99.2 ng/uL | |||
:Luc14 2, cleaned and sonicated: 122.2 ng/uL | |||
<br> | |||
Put samples at -20deg | |||
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Revision as of 08:10, 7 March 2016
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02/05/2016Cass purified and ran sonicated DNA on gel
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