Haynes Lab:Notebook/CRISPR Editing/2015/10/12: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==03//2015==
==10/12/2015==
 
Thawed Luc14
 
<br><br>
Ran PCR products for luc sequencing on gel, all worked but P160/238 was pretty faint. <br>
PCR purified with qiagen kit<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Label'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Seq primer'''
|-
| R1||P160/238||P283
|-
| R2||P160/238||P284
|-
| R3||P160/238||P285
|-
| R4||P160/238||P286
|-
| R5||P160/238||P287
|-
| R6||P161/233||P234
|-
| R7||P161/233||P219
|-
| R8||P161/233||P198
|-
| R9||P161/233||P169
|-
| R10||P161/233||P287
|-
| R11||P237/239||P236
|-
| R12||P237/239||P237
|-
| R13||P237/239||P238
|-
| R14||P237/239||P239
|}


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Latest revision as of 01:14, 27 September 2017

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10/12/2015

Thawed Luc14

Ran PCR products for luc sequencing on gel, all worked but P160/238 was pretty faint.
PCR purified with qiagen kit

Label Template Seq primer
R1 P160/238 P283
R2 P160/238 P284
R3 P160/238 P285
R4 P160/238 P286
R5 P160/238 P287
R6 P161/233 P234
R7 P161/233 P219
R8 P161/233 P198
R9 P161/233 P169
R10 P161/233 P287
R11 P237/239 P236
R12 P237/239 P237
R13 P237/239 P238
R14 P237/239 P239


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