Haynes Lab:Notebook/CRISPR Editing/2015/10/10: Difference between revisions
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Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing. | Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing. | ||
<br><br> | |||
Transfected gal4-eed open and closed chromatin with | |||
:750ng of g031 + 250ng of g031 EcoRI donor | |||
:750ng of g034 + 250ng of g034 EcoRI donor<br> | |||
antibiotic free media | |||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 08:13, 11 October 2015
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10/10/2015Purified PCR product g031 EcoR1 donor
Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing.
antibiotic free media | |||||||||
